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cell samples, the vials containing wet cells were frozen at − °c and then freeze dried for h (virtis sp scientific, ipswich, uk); these pellet samples corresponded to about mg of dry cells. in the case of astaxanthin extraction by supercritical co , l of yeast culture medium was collected after days
of bioreactor cultivation and centrifuged at × g for min (multifuge x r, fisher scientific, loughborough, uk). the pellet was washed twice with distilled water, frozen at − °c for h, and then freeze dried for h (virtis sp scientific, ipswich, uk) prior to scfe-co extraction. . enzymatic cell lysis and...
https://www.mdpi.com/2076-2607/8/3/430/htm