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inefficient, and often require troublesome and costly post-purification processing. as well, nanoplasmidstm are regulatory compliant (antibiotic-free), and the r k origin provides host-restricted replication, compared to traditional puc origin vectors that promiscuously replicate in gram negative host cells
. in summary, the nanoplasmidtm is an ideal non-viral vector for gene therapy of human cells and tissues, offering improved expression and duration, as well as excellent safety, ease of use, and economy of purification. today, nanoplasmidstm are being tested and used by more than biopharmaeutical organizations...
http://www.natx.com/vectors_cell-lines.html