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. materials and methods tissue culture hek- t cells (human embryonic kidney), mdck (madin-darby canine kidney) cells, a (adenocarcinomic human alveolar basal epithelial) cells, hela (human epitheloid cervix carcinoma) cells and hela hex atg knockout cells (nguyen, et al., ) were grown in dulbecco's modified
to ~ % confluency in appropriate flasks (t , t or t – sarstedt, numbrecht, germany) prior to splitting. once desired confluency was achieved, supernatant media was discarded, and cells were washed twice with warm pbs in order to remove residual culture media containing fbs. cells were then incubated...
https://kar.kent.ac.uk/79310/1/75Matthew%20D%20Badham%20PhD%20Thesis.pdf